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X-WR-CALNAME:Plant Proteoform Diversity
X-ORIGINAL-URL:https://snp2prot.uni-halle.de
X-WR-CALDESC:Events for Plant Proteoform Diversity
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DTSTART;TZID=Europe/Berlin:20250513T123000
DTEND;TZID=Europe/Berlin:20250513T140000
DTSTAMP:20260524T013614
CREATED:20250422T114450Z
LAST-MODIFIED:20250430T073909Z
UID:1658-1747139400-1747144800@snp2prot.uni-halle.de
SUMMARY:Colloquium with Jiorgos Kourelis (Imperial College London\, GB)
DESCRIPTION:On 13 May 2025\, Jiorgos Kourelis will present insights to his current research as part of the SNP2Prot Colloquium Series. \nSpeaker: Jiorgos Kourelis (Imperial College London\, GB)\nTitle: Pikobodies: What does it take to bioengineer NLR immune receptor-nanobody fusions \nDate: Tuesday\, 13 May 2025\nTime: 12:30 pm\nLocation: Lecture hall (GHS) of Biochemistry\, Kurt-Mothes-Straße 3 (Weinberg Campus)\, 06120 Halle (Saale) \n\nAbstract: Recurrent epidemics caused by plant pathogens pose a significant threat to global food security. While modifying natural components has been successful to retool the plant immune system\, this approach can be rapidly rendered ineffective by the emergence of new pathogen strains. Made-to-order synthetic plant immune receptors\, however\, offer a promising solution for tailored resistance to pathogen genotypes present in the field. Here we define four technical and conceptual advances which enabled successful engineering of NLR immune receptor-nanobody fusions\, termed Pikobodies\, for this purpose. These advances include 1)evolutionary analysis to identify domain boundaries\, 2) structural modelling to predict nanobody integration boundaries\, 3) rapid screening using transient immune and disease assays\, and 4) guided mutagenesis to abolish autoactivity triggered by some nanobody integrations. We show that plant immune receptors can serve as effective scaffolds for nanobodies that bind fluorescent proteins (FPs)\, and that the resulting immune receptor-nanobody fusions trigger immune responses and confer resistance against plant viruses expressing FPs. Since nanobodies can be raised against most molecules\, immune receptor-nanobody fusions have the potential to generate resistance against most plant pathogens and pests delivering effectors inside host cells. \n\nIf you like to meet with the speaker\, please contact the host\, Mariana Schuster. \n\nSee all SNP2Prot colloquia listed here: https://snp2prot.uni-halle.de/events/category/colloquium/ \nThe SNP2Prot Colloquium Series is integrated to the \n\nHalle Plant Science Colloquium (HPSC) – https://www.landw.uni-halle.de/prof/pflanzenernaehrung/hpsc/\nIPB Seminar Series – https://www.ipb-halle.de/forschung/symposien-und-kolloquien/vortraege/ipb-seminare
URL:https://snp2prot.uni-halle.de/event/colloquium-with-jiorgos-kourelis-imperial-college-london-gb/
LOCATION:Lecture hall (GHS) of Biochemistry (Weinberg Campus)\, Kurt-Mothes-Straße 3\, Halle (Saale\, 06120\, Germany
CATEGORIES:SNP2Prot Colloquia
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Berlin:20250429T123000
DTEND;TZID=Europe/Berlin:20250429T140000
DTSTAMP:20260524T013614
CREATED:20250214T134825Z
LAST-MODIFIED:20250422T090810Z
UID:1380-1745929800-1745935200@snp2prot.uni-halle.de
SUMMARY:Colloquium with Christina Weinberg (MLU\, General Biochemistry)
DESCRIPTION:On 29 April 2025\, Christina Weinberg will present insights to her current research as part of the SNP2Prot Colloquium Series. \nSpeaker: Christina Weinberg (MLU\, General Biochemistry)\nTitle: Unraveling biology using non-coding RNAs \nDate: Tuesday\, 29 April 2025\nTime: 12:30 pm\nLocation: Lecture hall (GHS) of Biochemistry\, Kurt-Mothes-Straße 3 (Weinberg Campus)\, 06120 Halle (Saale) \n\nAbstract: Non-coding RNAs (ncRNAs) have emerged as powerful regulators of cellular function\, extending far beyond their once-presumed roles as passive scaffolds or transcriptional noise. Among ncRNAs\, we study ribozymes – catalytic RNAs capable of self-cleavage – and riboswitches\, structured RNAs that sense specific metabolites to regulate gene expression. We aim to characterize their structural and biochemical properties\, unravel their biological roles\, where they are not known\, or leverage these RNAs as tools to manipulate gene regulatory circuits. In this talk\, I show the discovery and validation of new ncRNA classes and I will present our work on establishing an RNA-seq-technique to monitor ribozyme activity in cells that could be used to screen for new catalytic RNAs. Finally\, I will outline potential synergies with SNP2PROT and plant research initiatives at MLU. \n\nDownload PDF \nIf you like to meet with the speaker\, please contact the host\, Marcel Quint or Christina Weinberg directly. \n\nSee all SNP2Prot colloquia listed here: https://snp2prot.uni-halle.de/events/category/colloquium/ \nThe SNP2Prot Colloquium Series is integrated to the \n\nHalle Plant Science Colloquium (HPSC) – https://www.landw.uni-halle.de/prof/pflanzenernaehrung/hpsc/\nIPB Seminar Series – https://www.ipb-halle.de/forschung/symposien-und-kolloquien/vortraege/ipb-seminare
URL:https://snp2prot.uni-halle.de/event/colloquium-with-christina-weinberg-mlu-general-biochemistry/
LOCATION:Lecture hall (GHS) of Biochemistry (Weinberg Campus)\, Kurt-Mothes-Straße 3\, Halle (Saale\, 06120\, Germany
CATEGORIES:SNP2Prot Colloquia
ORGANIZER;CN="Marcel Quint":MAILTO:marcel.quint@landw.uni-halle.de
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Berlin:20250408T170000
DTEND;TZID=Europe/Berlin:20250408T183000
DTSTAMP:20260524T013614
CREATED:20250214T135432Z
LAST-MODIFIED:20250325T090026Z
UID:1388-1744131600-1744137000@snp2prot.uni-halle.de
SUMMARY:Colloquium with Holger Puchta (KIT\, Karlsruhe)
DESCRIPTION:On 8 April 2025\, Holger Puchta (KIT\, Karlsruhe) will present insights to his current research as part of the SNP2Prot Colloquium Series. \nSpeaker: Holger Puchta (KIT\, Karlsruhe)\nTitle: Applying CRISPR/Cas to plants: From gene editing to genome restructuring \nDate: Tuesday\, 8 April 2025\nTime: 5 pm\nLocation: Lecture hall E.02\, Theodor-Lieser-Strasse 9 (Heide Süd Campus)\, 06120 Halle (Saale) \n\nAbstract: Till today programmable nucleases as CRISPR/Cas have been applied to plants mainly on genes for the improvement of traits. However\, breeding also requires the breaking or establishing genetic linkages on the chromosome level. Recent improvements in sequence analysis of crop plants reveal that multi Mb long inversions occur with high frequency between different genotypes\, leading to crossover suppression. We were not only able to demonstrate that inversions up to almost chromosome size can be achieved in Arabidopsis thaliana by application of CRISPR/Cas\, but also meiotic recombination can be redirected this way. Thus\, on one side a recombination dead region could be reactivated after 5000 years and on the other almost a complete chromosome could be excluded from genetic exchange. We also induced inversions of serval Mbs to move pericentric heterochromatic regions into euchromatic chromosome arms. Interestingly\, we could not detect any significant change in chromatin state nor expression patterns in the next generations. Using Cas9\, we were also able to induce heritable translocations in the Mb range between heterologous chromosomes in Arabidopsis. Again\, we found robust conservation of the transcriptome and the epigenome to such changes. This indicates that we should be able to restructure plant genomes on an even more global scale. Some recent results sustaining this hypothesis will be presented. \n\nDownload PDF \nIf you like to meet with the speaker\, please contact the host\, Sascha Laubinger in advance. \nThe SNP2Prot Colloquium Series is integrated to the \n\nHalle Plant Science Colloquium (HPSC) – https://www.landw.uni-halle.de/prof/pflanzenernaehrung/hpsc/\nIPB Seminar Series – https://www.ipb-halle.de/forschung/symposien-und-kolloquien/vortraege/ipb-seminare
URL:https://snp2prot.uni-halle.de/event/colloquium-with-holger-puchta-kit-karlsruhe/
LOCATION:Lecture hall E.02 (Heide Süd Campus)\, Theodor-Lieser-Strasse 9\, Halle (Saale)\, 06120
CATEGORIES:SNP2Prot Colloquia
ORGANIZER;CN="Sascha Laubinger":MAILTO:sascha.laubinger@genetik.uni-halle.de
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Berlin:20250311T123000
DTEND;TZID=Europe/Berlin:20250311T140000
DTSTAMP:20260524T013614
CREATED:20250214T135802Z
LAST-MODIFIED:20250228T102735Z
UID:1398-1741696200-1741701600@snp2prot.uni-halle.de
SUMMARY:Colloquium with Roman Kouril (Palacky University Olomouc\, Czech Republic)
DESCRIPTION:On 11 March 2025\, Roman Kouril (Palacky University Olomouc\, Dept. of Biophysics) will present insights to his current research as part of the SNP2Prot Colloquium Series. \nSpeaker: Roman Kouril (Palacky University Olomouc\, Dept. of Biophysics)\nTitle: Mimicking the spruce-type photosystem II supercomplex in Arabidopsis thaliana \nDate: Tuesday\, 11 March 2025\nTime: 12:30 pm\nLocation: Lecture hall (GHS) of Biochemistry\, Kurt-Mothes-Straße 3 (Weinberg Campus)\, 06120 Halle (Saale) \n\nAbstract: Photosystem II (PSII) is a photosynthetic pigment-protein supercomplex consisting of a core and variable number of light-harvesting complexes (LHCII). Current knowledge of structure of green plant PSII supercomplexes comes from evolutionary distant representatives of green algae and flowering plants (angiosperms) and it points number of similar but also distinct features reflecting their position in the evolutionary tree. To make this view more complete\, we performed a structural analysis of the PSII supercomplex from Norway spruce\, a representative of gymnosperms\, using single particle cryo electron microscopy. The solved structure of spruce PSII supercomplex at 2.8 Å resolution\, which allowed us its detailed structural-functional comparison with photosystems from other plant species. We identified specific properties of spruce photosystem II that distinguish it from PSII of other land plants. To explore the potential physiological benefits of these unique properties\, we prepared and studied various Arabidopsis thaliana mutants in an attempt to mimic the spruce-type PSII supercomplex. The results of ongoing research are gradually leading us to identify key features that are important for its formation. \n\nDownload PDF \nIf you like to meet with the speaker\, please contact the host\, Panagiotis Kastritis in advance. \nThe SNP2Prot Colloquium Series is integrated to the \n\nHalle Plant Science Colloquium (HPSC) – https://www.landw.uni-halle.de/prof/pflanzenernaehrung/hpsc/\nIPB Seminar Series – https://www.ipb-halle.de/forschung/symposien-und-kolloquien/vortraege/ipb-seminare
URL:https://snp2prot.uni-halle.de/event/colloquium-with-roman-kouril-palacky-university-olomouc-czech-republic/
LOCATION:Lecture hall (GHS) of Biochemistry (Weinberg Campus)\, Kurt-Mothes-Straße 3\, Halle (Saale\, 06120\, Germany
CATEGORIES:SNP2Prot Colloquia
ORGANIZER;CN="Panagiotis Kastritis":MAILTO:panagiotis.kastritis@bct.uni-halle.de
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Berlin:20250120T150000
DTEND;TZID=Europe/Berlin:20250120T170000
DTSTAMP:20260524T013614
CREATED:20250214T140104Z
LAST-MODIFIED:20250422T091024Z
UID:1401-1737385200-1737392400@snp2prot.uni-halle.de
SUMMARY:Colloquium with Ute Krämer (Ruhr University Bochum)
DESCRIPTION:On 20 January 2025\, Ute Krämer will present insights to her current research as part of the SNP2Prot Colloquium Series. \nSpeaker: Ute Krämer (Ruhr University Bochum)\nTitle: “The genetic basis of adaptive physiological traits in the extremophile Arabidopsis halleri” \nDate: Monday\, 20 January 2025\nTime: 3:00 pm\nLocation: Lecture hall E.02\, Theodor-Lieser-Strasse 9 (Heide Süd Campus)\, 06120 Halle (Saale) \n\nAbstract: The extremophile metal hyperaccumulator Arabidopsis halleri is a diploid outcrossing stoloniferous perennial among the closest relatives of Arabidopsis thaliana. A. halleri is known for a vast phenotypic range of its leaf ionome\, with leaf Zn concentrations far above critical toxicity thresholds of ordinary plants\, and for its exceptionally broad edaphic range that includes soils containing toxic levels of heavy metals. Our objective is to unravel the genetic and physiological basis of phenotypic divergence both within A. halleri and by comparison to closely related species\, in an ecological context. I will briefly outline earlier insights related to metal homeostasis from cross-species comparative approaches and then sketch some recent further developments of this work. We established a biodiversity resource of ca. 1\,000 accessions of European A. halleri collected in the field\, which are edaphically and ionomically indexed\, that is\, leaf and soil elemental composition at the site of origin are known for each genotype. I will present results from QTL mapping using the F2 progeny of crosses of phenotypically contrasting parents\, and also from environmental association studies. Then I will show how haplotype-phased genome assemblies helped to identify structural variation at candidate loci. A subset of the identified genomic regions are under study towards the identification of causal genes and polymorphisms. Our results provide examples of the genetic architectures\, genomic alterations\, and gene functions underlying naturally selected extreme physiological traits in plants. At least in part\, the exceptionally large phenotypic range of A. halleri may reflect how plants utilize metals in mineral co-option for functions outside the nutritional requirements of the metalloproteome\, which are largely shared among. Our work provides insights into how plants cope with environmental change\, and can inform crop improvement and the development of plant-based technologies such as phytomining and phytoremediation. \n\nDownload PDF \nIf you like to meet with the speaker\, please contact the host\, Steffen Abel in advance. \nThe SNP2Prot Colloquium Series is integrated to the \n\nHalle Plant Science Colloquium (HPSC) – https://www.landw.uni-halle.de/prof/pflanzenernaehrung/hpsc/\nIPB Seminar Series – https://www.ipb-halle.de/forschung/symposien-und-kolloquien/vortraege/ipb-seminare
URL:https://snp2prot.uni-halle.de/event/20-jan-colloquium-with-ute-kramer-ruhr-university-bochum/
LOCATION:Lecture hall E.02 (Heide Süd Campus)\, Theodor-Lieser-Strasse 9\, Halle (Saale)\, 06120
CATEGORIES:SNP2Prot Colloquia
ORGANIZER;CN="Steffen Abel":MAILTO:Steffen.Abel@ipb-halle.de
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